Dr. Edwin Southern would have never thought that he would create a naming principle when he named a method for detection of a specific anti-cd34 antibody DNA sequence in DNA samples after his own name, as Southern blot.
Other blotting methods, including western blot, northern blot, eastern blot, and southwestern blot, are all named by the same principle, as they have similar procedures but with different experimental targets. To know about anti-cd34 antibody you can search the browser.
Western blot (also acknowledged as protein immunoblot), a technique utilized to detect particular proteins in a representation of tissue homogenate or extract, matters much in cell and molecular biology, as well as immunogenetics.
It has become the most commonly utilized method to detect protein properties, such as qualitative and quantitative detection of tissue antigens, the quality of peptide molecules, and the detection of virus antibodies or antigens.
Steps of western blotting:
» Tissue Preparation: Samples are taken from whole tissue or from cell culture
» Gel electrophoresis: Proteins of the sample are separated using gel electrophoresis which is used to resolve the problem of cross-reactivity of antibodies.
» Transfer: Separated proteins are moved from within the gel onto a membrane.
» Blocking: Steps must be taken to prevent the interactions between the membrane and the antibody used for detection of the target protein.
» Incubation: During this step, a reporter enzyme linked to a modified antibody would drive a colorimetric reaction and produce a color, when exposed to an appropriate substrate.
» Detection: The western blot is ready for detection of the probes that are labeled.